Furthermore, the inhibition of Rho kinase using Y27632 reduced TGF-β1 induced mCF differentiation, and TRPV4 antagonist AB155908 suppressed TGF-β1 induced Rho kinase activity, suggesting that the Rho kinase is downstream of TRPV4 in TGF-β1-induced CF differentiation. Here, TGFB1 is linked to cystic fibrosis.