Taken together, these findings suggest that TRPV4 regulates TGF-β1/mechanical force-induced fibroblasts differentiation through Rho/NOX4/MRTF-A (Figure 5) signaling molecules and identifies TRPV4 as a potential novel therapeutic target for regulating cardiac and pulmonary fibrosis. This evidence concerns the gene TRPV4 and pulmonary fibrosis.