In summary, VEGF, which is sequestered by high levels of sFLT-1 in preeclampsia, promotes both calcium-dependent and -independent (i.e., phosphorylation) stimulation of eNOS, whereas vasodilators such as acetylcholine signal through the Gαq pathway to increase endothelial calcium and activate eNOS [182]. Here, NOS3 is linked to preeclampsia.