While treatment with MTH1 inhibitor TH1579 alone did not significantly induce ROS formation after 24 h of treatment, combination of PLN and TH1579 led to increased ROS levels in NCI-H460 lung (Figure 3G,H) and T98G glioblastoma (Figure 3I,J) cancer cell lines. The gene discussed is PLN; the disease is glioblastoma.