Results from 3D culture assays, which reflect cancer cell stemness in anchorage-independent growth, indicate that palbociclib induced a significant inhibition of 3D colonies in MCF-7/C cells, whereas 3D colonies in palbociclib-treated MCF-7/FGFR1 cells were greater than the control cells under the same conditions in number, colony size (Figure 3A), and relative inhibition (Figure S3). The gene discussed is FGFR1; the disease is cancer.