In addition, low FTH levels and high TFR1 expression (as a typical pattern indicative of low iron needs) are displayed in cholangiocarcinoma cells grown in monolayers, whereas the opposite situation occurred when the same cholangiocarcinoma cell lines were allowed to form tumorspheres, accompanied with an increase in iron content and oxidative stress [43]. The gene discussed is TFRC; the disease is cholangiocarcinoma.