PFKFB3 and glioblastoma: Glioblastoma neurospheres exposed to a hypoxic environment show a 10-fold increased expression of PFKFB3, as well as upregulation of LDHA, pyruvate dehydrogenase kinase 1 (PDK1), and HK-2, via HIF-1α signaling, particularly within the necrotic core [11,12].