We queried the collective effect of the entire CHOL-hub gene set on tumor infiltrations of immune cells, and found that a higher GSVA score of CHOL-hub genes in CHOL tumor samples (Figure 6C) was positively correlated with higher tumor infiltrations of nTregs, iTregs, Th17 cells, central memory, MAIT, DCs, monocytes, macrophages, and Gamma-delta cells in CHOL patients, while negative corrections were observed between high GSVA scores of CHOL-hub genes and tumor infiltration by NKT, B-cells, CD4 and CD8, Tr1, and Tfh (Figure 6D). The gene discussed is CD8A; the disease is neoplasm.