MBNL1 and myotonic dystrophy type 1: An example of ASO application in DM1 is showed by Wheeler and colleagues, where the morpholino antisense oligonucleotide CAG25 was used to invade CUGexp hairpins to form a stable RNA-morpholino heteroduplex, blockings the formation of CUGexp-MBNL1 complexes (Figure 3A) [116].