In addition, AKF-D52 was observed to induce the translocation of cytochrome c and Smac/DIABLO from mitochondria to the cytosol, indicating that the disruption of ΔΨm leads to the cytosolic release of apoptosis-inducing mitochondrial proteins in NSCLC cells (Figure 3C). This evidence concerns the gene CYCS and non-small cell lung carcinoma.