In this study, we report that deslanoside reduced the cell proliferation rate, caused cell cycle arrest at G2/M, induced apoptosis, decreased colony formation, suppressed the migration and invasion abilities and inhibited tumor growth in human prostate cancer cell lines, including the AR-positive 22Rv1 and AR-negative PC-3 and DU 145 (Figure 1, Figure 2, Figure 3 and Figure 4). This evidence concerns the gene AR and Familial prostate cancer.