DCLK1 and renal cell carcinoma: To confirm the efficacy of DCLK1-IN-1, we performed Western blotting using a specific antibody to detect the phosphorylation of Serine 337 in the 82 kDa isoform of DCLK1 (Uniprot O15074-2; long-α) in three human RCC cell lines: ACHN, 786-O and CAKI-1.