Our findings in RCC demonstrate that DCLK1-IN-1 effectively inhibits the phosphorylation of DCLK1, downregulates key oncogenic and EMT targets (C-Met, C-Myc, and N-Cadherin), impairs colony formation, prevents cell migration and invasion, and inhibits RCC stemness. The gene discussed is DCLK1; the disease is renal cell carcinoma.