In order to assess the effect of DCLK1-IN-1 on immune-mediated tumor cell killing, we pretreated 786-O cells with 5 or 10 μM of DCLK1-IN-1 for 48 h, selected live cells via trypan blue exclusion, which were stained with fluorescent dye Calcein-AM and seeded into a 96 well plate in equal numbers. Here, DCLK1 is linked to neoplasm.