Similarly, Matrigel-based 3D spheroid assays showed promising properties for DCLK1-IN-1 against stemness in ACHN, 786-O, and CAKI-1 RCC cell lines (Figure 3C–E), and immunoblotting suggested a likely reduction in pluripotency (Figure 3A). The gene discussed is DCLK1; the disease is renal cell carcinoma.