To elucidate precisely how MCAK’s activity regulates the motility of cancer cells, using MCAK CRISPRi/a knockdown and overexpression cell lines, we show that interfering with the expression of MCAK causes a decreased cell motility and migration, which is attributed to an impaired FA turnover associated with compromised MT- and actin-cytoskeleton dynamics. The gene discussed is KIF2C; the disease is cancer.