We further investigated the immune cell infiltration levels of 24 immune cell types within PODNL1 high/low oligodendroglioma and astrocytoma separately for all four CpGs, and found that there was increased immune cell infiltration in the low methylation groups of these CpGs within both LGG subtypes (Supplementary Figure S7 and S8). The gene discussed is PODNL1; the disease is astrocytoma (excluding glioblastoma).