As the methylation status of specific PODNL1 CpGs showed strong associations with immune cell infiltration, we investigated the potential efficacy of immune checkpoint blockade (PD-L1, PD-1, and CTLA4) in groups of TCGA-LGG histological subtypes, astrocytoma and oligodendroglioma, based on PODNL1 expression and CpG methylation status (Figure 7). This evidence concerns the gene CD274 and astrocytoma (excluding glioblastoma).