It is possible that the observed unfavorable prognosis and tumor aggressiveness associated with low PODNL1 methylation/high PODNL1 expression is related to the enrichment of immune cell infiltration in the tumor microenvironment, as previous reports identified shorter glioma survival in patients with high immune infiltration [21,22,23,24,25]. Here, PODNL1 is linked to central nervous system cancer.