PRNP and prion disease: We finally demonstrated that wild-type (WT) mice intracerebrally inoculated with cell lysates from PrPSc-like PrP-producing N2aC24 cells developed prion disease, with abundant accumulation of PrPSc and spongiosis in their brains [24], indicating that, like authentic PrPSc produced by prion infection, PrPSc-like PrP produced in IAV/WSN-infected N2aC24 cells could form infectious prions.