Co-culture of GPC3+ tumor cells, MSCsGPC3-CD3 and T lymphocytes led to the increased production of IFN-γ in GPC3-specific CD4+T cells and the enhanced activation and expansion of GPC3-specific CTLs, which resulted in the efficient CTL-dependent killing of GPC3-expressing malignant cells. This evidence concerns the gene GPC3 and neoplasm.