All the differentially expressed MMPs showed statistical differences in promoter methylation between normal and colon cancer samples with a general tendency of hypomethylated promoters for upregulated MMPs and hypermethylated promoters for downregulated MMPs, while their mutation rate did not exceed 2.5%, except for MMP9, whose mutation rate was 11%. This evidence concerns the gene MMP9 and malignant colon neoplasm.