In line with a previously demonstrated activity of CBRs to activate the autophagy mechanism in vitro, another non-selective agonist for CB1R and CB2R, namely WIN55,212–2, showed a beneficial effect in inducing osteosarcoma cell death by the autophagy mechanism and was a potent adjuvant of other anticancer drugs, such as Adriamycin [61,125]. The gene discussed is CNR1; the disease is osteosarcoma.