Moreover, Type I NKT cell-deficient mice show reduced weight gain, lower plasma alanine transaminase (ALT) level, lower NAFLD activity score (NAS), reduced macrophages infiltration in the liver and downregulation of liver fibrosis markers including α-SMA, collagen type 1 alpha 1 (Col1a1), and collagen type 1 alpha 2 (Col1a2). Here, ACTA1 is linked to metabolic dysfunction-associated steatotic liver disease.