Next, we compared the levels of expression of genes coding for the proteins responsible for amyloid-β processing (APP, BACE1, BACE2, PSEN1, PSEN2, CD147, TMED10 and DYRK1A, RCAN1, CREB1) associated with Alzheimer’s disease, in the groups of TS21 and NK spheroids, and of the neural differentiation markers GFAP, PAX6 and MAPT (Figure 5). This evidence concerns the gene CREB1 and early-onset autosomal dominant Alzheimer disease.