Examination of the NKT cell subpopulation significantly elevated frequencies were measured in TIGIT+/CD226− and TIGIT−/CD226− subsets in EO preeclamptic cohort compared to healthy cohort; however, the frequency of the TIGIT−/CD226+ subpopulation was significantly decreased in EO preeclampsia (Figure 4D). Here, CD226 is linked to preeclampsia.