AKT1 and malignant colon neoplasm: Protein expression of the AMPK signaling pathway demonstrated that phospho-AMPKα (Thr172) and phospho-ACC (Ser79) levels significantly increased, whereas phospho-Akt (Ser473) and phospho-mTOR (Ser2448) levels decreased in BR colon cancer cells of HCT116, HT29, and SW480 treated with AICAR (Figure 6B).