SQSTM1 and breast carcinoma: To confirm that MAP1LC3B and SQSTM1 expressions were up-regulated due to autophagy inhibition, breast cancer cell lines (MCF7 and BT474) were treated with or without autophagy inhibitors [BafA1(100 nM), ConA (10 nM) and CQ (20μM)] for 5 h before harvesting.