To assess TRAP1 protective role by mPTP opening inhibition, Guzzo et al. performed a whole-cell Ca2+ retention capacity assay, which allows a quantitative assessment of mPTP induction, and reported that Trap1 knockdown increased mPTP sensitivity to Ca2+ in cancer cells, while Trap1 overexpression inhibited mPTP opening in mouse embryo fibroblasts (MEF) cells and protected SAOS-2 and MEF cells from mPTP opening in starvation conditions [6]. This evidence concerns the gene TRAP1 and cancer.