For instance, HPLC is not sufficient to discreetly detect variants as it is not sensitive and specific enough for an α-thalassaemia detection, especially in the presence of haemoglobin Constant Spring (non-deletion α-thalassaemia), indicated by the presence of a very tiny peak of Hb that is often missed [10,11]. Here, GSTM1 is linked to thalassemia.