Interlaboratory validation showed that the findings are reproducible in another laboratory setting with similar Unicel DxH 800, hence reiterating the usefulness of the αβ-algorithm in the screening of thalassemia cases, in particular, α and β-thalassemia in geographical locations with a high prevalence of thalassaemias such as Malaysia and other Asian countries. Here, PPIB is linked to thalassemia.