Lipotoxic stimulation affects downstream targets of cGAS-STING kinase, it induces the nuclear factor kappa beta (NF-kB) signaling to produce proinflammatory cytokines that activate macrophage to produce TGF-b1 and TNF-a which in turn stimulate hepatic stellate cells leading to liver fibrosis in NASH [8]. This evidence concerns the gene TNF and metabolic dysfunction-associated steatohepatitis.