MAP1LC3A and hepatocellular carcinoma: Furthermore, a statistical analysis showed that there was significant difference in LC3 puncta between the shWTAP cells and shCON cells, as well as the shWTAP cells and 3-MA-treated cells (Figure 1H), Taken together, the above results indicated that the knockdown of WTAP could facilitate the increased level of autophagy in HCC cells.