Previous studies have shown that gossypol induces cell death in cancer cells [18,19] and germline stem cells [20]; hence we used primary neonatal rat cardiomyocytes cultured in vitro and treated with GAA (20 and 50 μM) to assess the induction of cell death via flow cytometry analysis with Annexin V-FITC and PI for apoptosis and necrosis detection, respectively (Figure 4B). Here, ANXA5 is linked to cancer.