Quantification and statistical analyses of the expression levels of target proteins such as fibronectin, activated MMP9, vimentin, and Twist 1/2 from three independent experiments (see Section 4) revealed that change in activated MMP9 protein expression after 72 h of post-infection was indeed statistically significant (p-value < 0.05) in MDA-MB-231 cells (Figure 9, Panel A). Here, VIM is linked to infection.