Our results showed that serum LINC0094 and LINC00514 levels were significantly elevated in patients with chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC) compared with healthy controls, and the combined detection with alpha fetal protein (AFP) can significantly improve the diagnostic efficiency of liver diseases. This evidence concerns the gene AFP and laryngotracheoesophageal cleft.