In the current small sample study, the sensitivity of serum LINC00941 and LINC00514 in differential diagnosis of normal control and HCC patients were 85% and 90%, specificity was 86.67% and 56.67%, and the AUC reached 0.919 and 0.808, indicating that serum LINC00941 and LINC00514 had good diagnostic efficacy in the diagnosis of HCC; and when combined with AFP, they can significantly improve the sensitivity and accuracy of AFP diagnosis (87.5% vs. 60%, 75% vs. 60% and 0.962 vs. 0.815, 0.918 vs. 0.815). The gene discussed is AFP; the disease is hepatocellular carcinoma.