Although the clinicopathological features were the same in all leukemic mice (Supplementary Table 2), 2/3 CRISPRMLL-AF4+ mice had a CD19+CD10−CD20−IgM/IgD−CD34+/− proB ALL immunophenotype (Fig. 3e and Supplementary Fig. 5a), while the remaining mouse had a preB ALL immunophenotype, with the majority of cells being CD19+CD10+CD20−IgM/IgD−CD34− (Supplementary Fig. 5a, b). This evidence concerns the gene AFF1 and acute lymphoblastic leukemia.