To determine the molecular mechanism whereby Utx deficiency impaired brown fat thermogenesis and promoted diet-induced obesity, we performed RNA-seq analysis using iBAT from male UTXKO and fl/Y mice fed HFD diet for 12 weeks to unbiasedly examine gene expression pattern changes induced by Utx deficiency in brown fat. Here, KDM6A is linked to obesity due to melanocortin 4 receptor deficiency.