Moreover, in this study, we report that Act V exerts a greater inhibitory effect in the human CRC HCT-116 cell line than Act D, inducing apoptosis associated with the mitochondrial apoptotic pathway and the PI3K/AKT pathway synergistically, which enhances our comprehension of Act V’s mechanism and provides more detailed preclinical research data. Here, PIK3CA is linked to colorectal carcinoma.