Ag–Au nanorods have been functionalized with four different Raman-active molecules and conjugated with four kinds of antibodies (anti-epithelial cell adhesion molecule (anti-EpCAM), anti-CD44, anti-keratin, and an anti-insulin-like growth factor antigen (anti-IGF-I receptor β)) specific to breast cancer markers. This evidence concerns the gene EPCAM and breast carcinoma.