In the present study, it was confirmed that miR-577 targets LPR6 and β-catenin in pancreatic cancer cells, and LPR6 and β-catenin expression levels were positively regulated by FGD5-AS1, which helps to clarify the mechanism of the activation of the Wnt/β-catenin pathway in pancreatic cancer. Here, FGD5 is linked to familial pancreatic carcinoma.