Despite having nanomolar potency against SPHK1, it did not show promising anti-cancer effects in early study by Schnute et al. (2012) who demonstrated that PF-543 had insignificant effect on the cancer cell growth, when they tested it in cancer cell lines at single concentration of 1 μM (Schnute et al., 2012). The gene discussed is SPHK1; the disease is cancer.