The upregulation of miR-9 in CF cells downregulates its target anoctamin 1 (ANO1) alias calcium-activated chloride channel (transmembrane protein 16A, TMEM16A) and preventing the inhibition of ANO1 in vitro and in vivo CF models via the miR-9 target site blocker (TSB) elevates chloride efflux, mucociliary clearance, and migration rate of cells (Benedetto et al., 2017; Sonneville et al., 2017). The gene discussed is ANO1; the disease is cystic fibrosis.