RNA-Seq analysis indicated a downregulation of 603 and 2220 genes in Alk-F1178S;Th-MYCN and Rosa26_Alkal2;Th-MYCN tumours, respectively (log2 fold change of 1.5 at 1% FDR; Fig. 7a, b and Supplementary Dataset 1). This evidence concerns the gene MYCN and neoplasm.