Hierarchical clustering analysis using the Widmer and the Verfaillie gene sets confirmed that Wnt-3a treatment promoted the proliferative, differentiated melanoma cell phenotype, whereas TGFβ and siLATS1/2 treatment induced an invasive phenotype with a dominant effect over Wnt-3a treatment (Figs S16A and B and S17A and B). This evidence concerns the gene TGFB1 and melanoma.