In contrast, by using high-affinity anti-HA antibody and HA-tagged endogenous Tbx2 in melanoma, we were able to identify up to 7500 binding sites (clone 9, R2) with a robust set of sites identified in common between multiple replicates, of which ∼38% were within 3 kb of the transcription start site. This evidence concerns the gene TBX2 and melanoma.