For this, we chose the B16 mouse melanoma cell line because it is readily susceptible to senescence after Tbx2 depletion (Prince et al. 2004; Vance et al. 2005) and does not express Tbx3 (Vance et al. 2005), a highly related T-box factor expressed in many human melanoma cell lines (Rodriguez et al. 2008) that can potentially regulate similar genes to Tbx2 (Hoogaars et al. 2008). Here, TBX2 is linked to melanoma.