On this basis, we applied methods to induce autophagy in osteosarcoma cells, inhibit the autophagy process of osteosarcoma cells, and inhibit the phosphorylation level of mTOR by rapamycin to clarify the relationships among aloin, autophagy, the PI3K/AKT/mTOR axis and apoptosis. The gene discussed is MTOR; the disease is osteosarcoma.