Further interpretation of the 487 over eightfold genes showed that a total of 55/487 genes were shared in at least 3/5 profiles indicating their expressions were more convinced to be different in cancer comparing to normal tissues (Fig. 2F), and a large percent of these genes were collagen related regulators, for instance COL1A1, COL1A2, COL5A1, COL5A2, COL10A1 and COL11A1, which were associated with PAAD cancer related fibroblasts infiltration and directly affect patients recurrence free survival (Fig. 3A–F). Here, COL1A2 is linked to pancreatic adenocarcinoma.