We excluded dead cells and doublets from the flow cytometry analysis to detect exclusively live macrophages (CD45+CD11b+CD64+), neutrophils (CD45+CD11b+Ly6G+), monocyte-derived dendritic cells (CD45+CD11c+I-Ab+CD11b+Ly6Cint), monocyte undifferentiated macrophages (CD45+CD11c+IAb-/lowCD11b+Ly6Cint) and interstitial macrophages (CD45+CD11b+IAb+ CD6int/hi) that have active viral infection by assessing GFP reporter expression (data not shown). The gene discussed is PTPRC; the disease is viral infectious disease.