To obtain exogenous expression levels of BirA*-fused PAR6B similar to endogenous levels of PAR6B, we used a low dose of lentivirus virus (Multiplicity of Infection (MOI = 0.2) and used fluorescent activated cell sorting (FACS) to split cells into three groups based on GFP expression (lowest 25%, middle 50%, highest 25%). The gene discussed is PARD6B; the disease is infection.