To further investigate the consequences of the EoE-associated missense variants in DSP and PPL, we transduced EPC2 cells with constructs encoding non-variant and mutated variant DSP and PPL. Immunoblotting of the cell extracts showed that expression of all mutant DSP and PPL constructs were equivalent to that of the wild-type construct (Supplementary Fig. 9). Here, PPL is linked to eosinophilic esophagitis.