As shown in Figure 4, after treatment with 100ng/ml exogenous HMGB1, the western blot results showed the activation of p-GSK 3β and the up-regulation of N-CA, Bcl-2, and Ki67 in response to HMGB1 stimulation, while E-CA expression was down-regulated in pancreatic cancer cells in response to HMGB1 stimulation. Here, MKI67 is linked to familial pancreatic carcinoma.