To investigate whether the KLF10/EGFR axis plays a dynamic role in the process of PDT reversing cholangiocarcinoma gemcitabine resistance, we co-transfected RBE-R cells with si-KLF10 and si-EGFR, exposed the cells to PDT treatment, and examined for the mRNA expression of KLF10 and EGFR. This evidence concerns the gene EGFR and cholangiocarcinoma.