Down-regulated trophoblast pathways in response to mTORC2 inhibition included alanine, aspartate and glutamate metabolism, carbohydrate digestion and absorption, DNA replication, drug metabolism, leukocyte trans-endothelial migration, metabolic pathways, N-Glycan biosynthesis, neurotrophin signaling pathway, nitrogen metabolism, nucleotide excision repair, pyrimidine metabolism, toll-like receptor signaling pathway, type I diabetes mellitus, type II diabetes mellitus, and vitamin B6 metabolism (Table 4). The gene discussed is BDNF; the disease is type 1 diabetes mellitus.