We chose some regulators for RT-qPCR validation, and the qPCR results revealed that METTL3, METTL14, and FTO had consistently low expression in 33 paired samples with TCGA data, while YTHDF1 and YTHDF3 showed increased expression in tumor samples compared to adjacent tissues (Figure 2C). The gene discussed is METTL3; the disease is neoplasm.