Overexpression of miR-33b-3p led to deceased expression of DOCK4 in both mRNA and protein levels in highly metastatic PC-3M-1E8 cells, while inhibition of miR-33b-3p could enhance the expression of DOCK4 in lowly metastatic PC-3M-2B4 cells (Figures 5B, C). This evidence concerns the gene DOCK4 and pachyonychia congenita.