Meanwhile, ROC curve analysis revealed that all the five neddylation pathway components, especially UBE2M, had a good diagnostic value for HCC (AUC: NEDD8 = 0.963, NAE1 = 0.920, UBA3 = 0.833, UBE2M=0.968, and RBX1 = 0.922) (Figure 1B). This evidence concerns the gene NAE1 and hepatocellular carcinoma.