To further validate the neddylation pathway as a promising anti-tumor target, this study investigated the relationship between activated neddylation pathway (NEDD8-UBE2M-RBX1 axis) and immune cell infiltration by analyzing mRNA transcriptome and clinical data of HCC samples from The Cancer Genome Atlas (TCGA) database. The gene discussed is UBE2M; the disease is hepatocellular carcinoma.